Enzyme immunoconjugates utilizing glucose oxidase and myeloperoxidase are cytotoxic to Candida tropicalis

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Enzyme immunoconjugates utilizing glucose oxidase and myeloperoxidase are cytotoxic to Candida tropicalis.

A dual-enzyme immunoconjugate system was evaluated for its cytotoxic effect on Candida tropicalis. Glucose oxidase, which generates hydrogen peroxide in the presence of glucose and oxygen and myeloperoxidase, which catalyzes the oxidation of halides in the presence of hydrogen peroxide, were each conjugated to a C. tropicalis-specific monoclonal antibody. Neither the glucose oxidase nor the mye...

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Absence of photoreactivating enzyme in Candida albicans. Candida stellatoidea, and Candida tropicalis.

In vitro assays demonstrate photoreactivating enzyme activity in extracts of Candida pseudotropicalis but not in extracts of Candida albicans, Candida stellatoidea, or Candida tropicalis.

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Emergence of Candida tropicalis resistant to caspofungin.

Sir, Candida species is the fourth leading cause of nosocomial bloodstream infection in the USA. The incidence and mortality of invasive candidiasis (IC) remain high despite new antifungal agents. Although Candida albicans remains the most common isolated species causing IC, the incidence of IC caused by non-albicans species is increasing. Caspofungin, an echinocandin, is approved for treatment...

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Non-albicans Candida species are growing in prominence in industrial biotechnology due to their ability to utilize hemicellulose. Here, we present the draft genome sequences of an inhibitor-tolerant Candida tropicalis strain (Y6604) and Candida boidinii NCAIM Y01308T.

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A method for the preparation of protoplasts from Candida tropicalis cultivated on n-tetradecane is described. This essentially consists of replacing the mannitolsorbitol solution of the classical helicase technique by 1 M magnesium sulfate and lowering the pH to 4.1 during incubation in the presence of helicase. The protoplasts thus prepared behave like intact cells and are capable of consuming...

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ژورنال

عنوان ژورنال: Antimicrobial Agents and Chemotherapy

سال: 1990

ISSN: 0066-4804,1098-6596

DOI: 10.1128/aac.34.5.875